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Image Search Results
Journal: Frontiers in Neuroscience
Article Title: MRIES: A Matlab Toolbox for Mapping the Responses to Intracranial Electrical Stimulation
doi: 10.3389/fnins.2021.652841
Figure Lengend Snippet: Main GUI of the toolbox. (A) Data processing GUI, including the processing pipeline, separate processing step, and visualization. (B) Visualization GUI. The individual brain is shown in different views. The lower right part shows the information and controllers highlighted in the color-coded box. The volume and voxel coordinates are illustrated in the blue box. The subject information is illustrated in the pink box. The electrode information is illustrated in the yellow box. The specific stimulated electrode pair and indicator illustrated in the green box can be chosen and then the responses to this electrode pair are shown in the volume space. Other visualization GUIs and 3D-electrode locations are shown in the red box.
Article Snippet: The set of the tools are easily available by
Techniques:
Journal: eLife
Article Title: Sloppy morphological tuning in identified neurons of the crustacean stomatogastric ganglion
doi: 10.7554/eLife.22352
Figure Lengend Snippet: Neurons were identified physiologically and then filled with Lucifer yellow. Following fixation and immunohistochemical amplification with a rabbit anti-Lucifer yellow primary antibody and an Alexa Fluor 488-tagged secondary antibody, each dye-fill was imaged at 63x magnification as a series of z-stacks that tiled the neuronal structure across the x-y plane (z-stacks contained slices at increments of ~0.5 µm). These z-stacks were stitched together with custom software written in MATLAB. The image stack of each full three-dimensional neuronal structure was then manually traced (image shown is a screenshot from the KNOSSOS platform). The three-dimensional skeletal structures were then converted to hoc files that could be analyzed with a custom quantitative morphology toolbox composed in Python. All conversion and analytical scripts are freely available on the Marder Lab Github repository. DOI: http://dx.doi.org/10.7554/eLife.22352.024
Article Snippet: These tile stacks were aligned and stitched with a
Techniques: Immunohistochemical staining, Amplification, Software